Blz1

Dna Structure And Sequence Of The Blz1 Gene From Barley A Schematic The blz1 gene was used in a blast search on the triticum aestivum genome and three homoeologous sequences on chromosome 5 were retrieved. the coding sequences (cds) of the a, b and d shp copies encoded polypeptides of 392, 400 and 406 amino acid residues, respectively, which include the characteristic dna binding domain of the bzip class of tfs. The blz1 mrna was detected early in endosperm development and was also expressed in roots and leaves. the blz1 protein was a potent transcriptional activator in a yeast system; 85% of its activity was associated with the first 203 amino acid residues at the n terminus, which included two acidic regions.

Transactivation Capacity Of Blz1 In Yeast A Schematic Structures Of The coexistence ofblz2 and blz1 mrnas in the barley endosperm prompted us to test if blz2 and blz1 may form heterodimers in vivo. we explored this possibility by the yeast two hybrid system, using as a “bait” the bzip domain of blz2, fused to the gal4dbd and as a “prey” the whole blz1 or different fragments of it fused to the gal4ad. The regulation network has been extensively studied in barley where blz1 and blz2, members of the basic leucine zipper (bzip) family, activate the synthesis of hordeins. to date, in wheat, only the ortholog of blz2, storage protein activator (spa), has been identified as playing a major role in the regulation of gsp synthesis. The blz1 mrna was detected early in endosperm development and was also expressed in roots and leaves. the blz1 protein was a potent transcriptional activator in a yeast system; 85% of its activity. Gtgagtcat for barley blz1 and blz2 . ccacgtgg and tgacgtca for cprf2 : n terminal hydrophobic or acidic signature (activation domain), ser thr cluster acidic aa stretch. putative phosphorylation sites. d: 7, 8, 10–13: 325–481: n terminal: 3 plus g at position 4: tga3, 2 and 5 with npr1 , tga4 with atebp : tgacgt g for tga1–6 : none: e: 4.

Blz1 To This Heat Album By Blz1 Spotify The blz1 mrna was detected early in endosperm development and was also expressed in roots and leaves. the blz1 protein was a potent transcriptional activator in a yeast system; 85% of its activity. Gtgagtcat for barley blz1 and blz2 . ccacgtgg and tgacgtca for cprf2 : n terminal hydrophobic or acidic signature (activation domain), ser thr cluster acidic aa stretch. putative phosphorylation sites. d: 7, 8, 10–13: 325–481: n terminal: 3 plus g at position 4: tga3, 2 and 5 with npr1 , tga4 with atebp : tgacgt g for tga1–6 : none: e: 4. Blz1, contained six exons and five introns, plus a 442 nt long 59 untranslated leader sequence, and was located on chromosome 5h. the blz1 mrna was detected early in. Barley blz2, a seed specific bzip protein that interacts with blz1 in vivo and activates transcription from the gcn4 like motif of b hordein promoters in barley endosperm j. biol. chem. (1999).

Transactivation Of The Barley Itr1 Promoter By Blz1 In Developing Blz1, contained six exons and five introns, plus a 442 nt long 59 untranslated leader sequence, and was located on chromosome 5h. the blz1 mrna was detected early in. Barley blz2, a seed specific bzip protein that interacts with blz1 in vivo and activates transcription from the gcn4 like motif of b hordein promoters in barley endosperm j. biol. chem. (1999).