Methylene Blue Ctab Agar Test A And Blood Hemolysis Test B

Methylene Blue Ctab Agar Test A And Blood Hemolysis Test B Download scientific diagram | methylene blue ctab agar test (a) and blood hemolysis test (b). biosurfactant producing strains produce a halo when grown in a solid medium of defined composition. The ctab agar plate method is a semi quantitative assay for the detection of extra cellular glycolipids or other anionic surfactants. it was developed by siegmund and wagner. 54 the microbes of interest are cultivated on a light blue mineral salts agar plate containing the cationic surfactant cetyltrimethylammonium bromide and the basic dye.

Methylene Blue Ctab Agar Test A And Blood Hemolysis Test B For screening, microbial isolates are spot inoculated on mineral salt agar medium supplemented with 0.005 g l methylene blue and 0.2 g l ctab and grown for 1 to 2 days. formation of the clear blue zone surrounding the streaks on dark blue agar plates is attributed to the secretion of anionic biosurfactant. Ctab agar assay. ctab agar plates were prepared by adding 0.15 g of cetyltrimethylammonium bromide (ctab; sigma aldrich, oakville, on, canada), 0.005 g methylene blue (sigma aldrich) and 12 g of agar to 1 l of distilled water, adjusted to ph 7 and sterilized (tahzibi et al., 2004). two holes (6.5 mm diameter) were made in the ctab plates, and. The positive detection of biosurfactants on agar containing 0.5 mg ml ctab and 0.2 mg ml methylene blue is seen by the formation of a blue halo surrounding a bacterial colony or sample (fig. 8). the blue halo is formed through the binding and forming of a complex of anionic surfactant with the cationic surfactant of ctab. The underlying principle is that glycolipids, such as rhamnolipids, have a nonpolar tail and polar head groups, which form insoluble ion pairs with synthetic cationic surfactant ctab and precipitate appearing as a dark blue halo. isolates to be tested are streaked on mineral salt agar medium containing 0.2 g l ctab and 0.005 g l methylene blue.

Ctab Methylene Blue Agar Method For The Detection Of Surfactant In The The positive detection of biosurfactants on agar containing 0.5 mg ml ctab and 0.2 mg ml methylene blue is seen by the formation of a blue halo surrounding a bacterial colony or sample (fig. 8). the blue halo is formed through the binding and forming of a complex of anionic surfactant with the cationic surfactant of ctab. The underlying principle is that glycolipids, such as rhamnolipids, have a nonpolar tail and polar head groups, which form insoluble ion pairs with synthetic cationic surfactant ctab and precipitate appearing as a dark blue halo. isolates to be tested are streaked on mineral salt agar medium containing 0.2 g l ctab and 0.005 g l methylene blue. Ctab agar plate method detection of crude biosurfactant. ctab agar plate strategy is a semiquantitative assay for the identification of anionic surfactants (thavasi et al. 2011). blue agar plates contain cetyltrimethylammonium bromide (ctab 0.2 mg ml), methylene blue (mb 0.5 mg 100 ml, basic dye) and glucose (2% v v). The composition of ctab methylene blue agar medium was (g l): glucose 20, peptone 10, beef powder 1, ctab 0.78, methylene blue 0.002, yeast extract 0.5, agar 17, ph 7.2 in 1,000 ml distilled water. then the halo of the colonies was extracted, streak on selective ctab methylene blue agar medium and cultivated at 37 °c for 9 d.

Ctab Methylene Blue Agar Method For The Screening Of Surfactant Ctab agar plate method detection of crude biosurfactant. ctab agar plate strategy is a semiquantitative assay for the identification of anionic surfactants (thavasi et al. 2011). blue agar plates contain cetyltrimethylammonium bromide (ctab 0.2 mg ml), methylene blue (mb 0.5 mg 100 ml, basic dye) and glucose (2% v v). The composition of ctab methylene blue agar medium was (g l): glucose 20, peptone 10, beef powder 1, ctab 0.78, methylene blue 0.002, yeast extract 0.5, agar 17, ph 7.2 in 1,000 ml distilled water. then the halo of the colonies was extracted, streak on selective ctab methylene blue agar medium and cultivated at 37 °c for 9 d.