Guided by the cocrystal structure of 5, sar exploration revealed that the corresponding urea compound 7 exhibited similar potency with an improved solubility profile. these studies also indicated that a 3 pyridyl group was the preferred substituent at one inhibitor terminus and also identified a urea moiety as the optimal linker to the remainder of the inhibitor structure. Structure based discovery of novel amide containing nicotinamide phosphoribosyltransferase (nampt) inhibitors rystals were grown from 0.2ul 0.2ul drops.
Nicotinamide phosphoribosyltransferase1,2 ethanediolethylene glycoln {4 [(3,5 difluorophenyl)sulfonyl]benzyl}imidazo[1,2 a]pyridine 6 carboxamidephosphate ion. Crystal structures of several urea and thiourea derived compounds in complex with the nicotinamide phosphoribosyltransferase (nampt) protein were utilized to design a potent amide containing inhibitor bearing an aza indole moiety (7, nampt bc ic50 = 9.0 nm, a2780 cell proliferation ic50 = 10 nm). the nampt–7 cocrystal structure was subsequently obtained and enabled the design of additional. 4kfn: structure based discovery of novel amide containing nicotinamide phosphoribosyltransferase (nampt) inhibitors rcsb pdb, pdb structures, and computed. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. the rcsb pdb also provides a variety of tools and resources. users can perform simple and advanced searches based on annotations relating to sequence, structure and function. these molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
4kfn: structure based discovery of novel amide containing nicotinamide phosphoribosyltransferase (nampt) inhibitors rcsb pdb, pdb structures, and computed. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. the rcsb pdb also provides a variety of tools and resources. users can perform simple and advanced searches based on annotations relating to sequence, structure and function. these molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. In the development of amide containing b3gnt2 inhibitors guided by structure based drug design, imidazolones were found to successfully serve as amide bioisosteres. this novel imidazolone isosteric strategy alleviated torsional strain of the amide bond on binding to b3gnt2 and improved potency, isoform selectivity, as well as certain physicochemical and pharmacokinetic properties. Data collection and structure refinement statistics are summarized in table s1. the structure was solved by molecular replacement (mr) with known nampt structure (pdb code:3dhf) as the search model using the program phaser. the structure was further refined using program refmac5 (murshudov, g. n.; et al., acta crystallogr. d biol. crystallogr.
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