What Is Blood Agar Media Complete Clear Overview Youtube Hello viewers !!!my name is kavindu lakmal , medical laboratory science student from university of peradeniya. i designed this video from my text books not. Blood agar composition, principle, preparation, uses and.
Blood Agar Composition Preparation Uses Lab Tests Guide Blood agar composition, preparation, uses and pictures. Suspend 40.5 grams in 1000 ml distilled water or deionized water. heat to boiling to dissolve the medium completely. sterilize by autoclaving at 15 lbs pressure (121°c) for 15 minutes. cool to 45 50°c and aseptically add 7% sterile sheep blood. mix well and pour into sterile petri plates. Before inoculation, a blood agar plate appears red and opaque due to the large particulate red blood cells (rbcs) contained within. if an organism growing on the plate produces beta (β) hemolysins, the rbcs are completely destroyed or lysed and the medium surrounding the growth loses its opacity. if this is the case, the medium may become. Autoclave for about 15 minutes at 121 degree celsius. transfer the blood agar base to the water bath at a temperature of 50°c. once the base has cooled down, start adding sterile blood agar (5% of defibrinated blood) and mix well to prevent the formation of air bubbles. dispense about 15 ml to a sterile petri plate.
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